Solution Information | help | |
Enzyme: | Serine/threonine-protein kinase Chk1 | |
inhibitor: | BDBM14717 | |
substrate: | Cdc25C peptide substrate | |
Solution Type: | Aqueous | |
pH at Preparation: | 7.4 | |
Temp. Prep.: | 22 | |
Comments: | n/a | |
Solute 1: | DTT | |
Concentration: | 0.5 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 2: | MgCl2 | |
Concentration: | 10 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 3: | Triton X-100 | |
Concentration: | 0.08 mM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solute 4: | ATP | |
Concentration: | 5 uM | |
Concentration: | n/a | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solvent 1: | water | |
Concentration: | 1.0 | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
Solvent 2: | DMSO | |
Concentration: | 2% | |
Purity: | n/a | |
Purification Meth.: | n/a | |
Comments: | n/a | |
pH Buffer 1: | HEPES | |
Concentration: | 25 mM | |
ΔHion: | n/a | |
Comments: | n/a | |
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